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目的建立三七破壁粉粒的含量测定方法。方法利用Waters高效液相色谱仪测定三七破壁粉粒的三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量,流动相为乙腈-水,流速为1.0 mL.min-1,测定波长为203nm。结果三七皂苷R1在0.22～2.19μg范围内线性关系良好,平均回收率为103.12%,RSD值为1.44%;人参皂苷Rg1在0.79～7.90μg范围内线性关系良好,平均回收率为102.23%,RSD值为1.97%;人参皂苷Rb1在0.85～8.53μg范围内线性关系良好,平均回收率为96.63%,RSD值为0.99%。结论此法简便、快速,适用于三七破壁粉粒的含量测定,为进一步研究三七破壁粉粒的质量打下了基础。
Objective To establish a method for the determination of the content of Panax notoginseng powder. Methods The content of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Panax notoginseng granules was determined by Waters HPLC. The mobile phase consisted of acetonitrile-water and the flow rate was 1.0 mL.min-1. The detection wavelength was 203nm. Results The notoginsenoside R1 had a good linearity in the range of 0.22-2.19 μg with an average recovery of 103.12% and a RSD value of 1.44%. Ginsenoside Rg1 showed good linearity in the range of 0.79-7.90 μg with an average recovery of 102.23% RSD value was 1.97%. Ginsenoside Rb1 showed good linearity in the range of 0.85 ~ 8.53μg with an average recovery of 96.63% and a RSD value of 0.99%. Conclusion This method is simple and rapid, suitable for determination of the content of Panax notoginseng powder, which laid the foundation for further study on the quality of Panax notoginseng powder.