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利用大肠杆菌表达表皮生长因子受体胞外段(ED-EGFR),将目的基因片段插入到pET28a载体中,将插入目的基因载体转化入BL21表达.利用异丙基-β-D-硫代半乳糖苷(ITPG)诱导BL21表达ED-EGFR,破碎后80％的ED-EGFR为可溶性表达,其他则存在包涵体中,将菌液离心上清通过镍柱亲和层析纯化,纯化蛋白样品经过胶浓度为12％ SDS-PAGE电泳,条带在相对分子质量58 k左右出现单一条带,并且纯度达到95％以上.通过BCA检测蛋白浓度,利用CCK8方法检测ED-EGFR具有较高活性,人肺非小细胞癌细胞(H1648细胞)增殖明显被抑制,并且在浓度320 ng/mL下抑制效果最好.“,”The target gene fragment was inserted into the pET28a vector.The target gene vector was inserted into BL21 to express the extracellular fraction of epidermal growth factor receptor (ED-EGFR).The expression of ED-EGFR in BL21 was induced with isopropyl-3-D-thiogalactoside (ITPG).80％ of ED-EGFR was expressed as soluble after disintegration.Others were existing in inclusion bodies.The purity of the purified protein sample was 12％ by SDS-PAGE.The band was a single band at about 58 ku,and the purity was over 95％.The proliferation of human lung non-small cell carcinoma cells (H1648 cells) was inhibited by CCK8 method,and the inhibitory effect was the best at the concentration of 320 ng/mL.