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目的 :探讨血管紧张素转换酶 ( ACE)基因的插入 /缺失 ( insertion/deletion,I/D)多态性与冠状动脉病变的相关性。方法 :应用聚合酶链反应 ( PCR)扩增技术检测 86例行冠状动脉造影患者的 ACE基因 I/D多态性。结果 :冠状动脉异常组的 DD基因型频率 0 .41,D等位基因频率 0 .5 2 ,显著高于冠状动脉正常组的 0 .15和 0 .2 9( P <0 .0 5 ) ;DD基因型与冠状动脉病变有关 ( OR=3.97,P<0 .0 5 )。多支病变与 DD基因型的关系更为密切 ( OR=4.72 ,P<0 .0 5 )。冠状动脉正常组、单支病变组和多支病变组的 DD型频率依次为 0 .15、0 .33和 0 .46( P <0 .0 5 ) ,D等位基因频率为 0 .2 9、0 .44和 0 .5 6( P <0 .0 1)。结论 :ACE基因的 I/D多态性与冠状动脉病变及其严重程度相关 ,DD型及 D等位基因频率随冠状动脉病变及其程度的加重而逐渐升高。在冠状动脉病变患者中 DD及 ID型患者的吸烟率、甘油三酯及血压显著低于 型者 ( P <0 .0 5 ) ,说明 D等位基因及 DD基因型可能是冠心病低危人群冠状动脉病变的重要危险因素之一。
Objective: To investigate the relationship between angiotensin converting enzyme (ACE) gene insertion / deletion (I / D) polymorphism and coronary artery disease. Methods: Polymerase chain reaction (PCR) amplification was used to detect ACE gene I / D polymorphism in 86 patients undergoing coronary angiography. Results: The frequencies of DD genotypes in patients with abnormal coronary artery were 0.41, and the frequency of D allele was 0.52, which was significantly higher than that of normal coronary artery patients (0. 15 and 0.29) (P <0.05). The DD genotype was associated with coronary artery disease (OR = 3.97, P <0.05). Multivariate lesions and DD genotypes were more closely related (OR = 4.72, P <0.05). The frequency of DD type in normal coronary artery group, single vessel lesion group and multiple vessel lesion group were 0 .15, 0 .33 and 0. 46 (P <0. 05) and D allele frequency was 0.29 , 0 .44 and 0 .5 6 (P <0 .0 1). Conclusion: The I / D polymorphism of ACE gene is correlated with the severity of coronary artery disease and the severity of coronary artery disease. The frequencies of DD and D allele increase with the severity of coronary artery disease. The prevalence of smoking, triglyceride and blood pressure in patients with DD and ID were significantly lower than those in patients with coronary artery disease (P <0.05), indicating that the D allele and DD genotype may be at low risk of coronary heart disease One of the important risk factors of coronary artery disease.