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我国于1975年已利用马铃薯茎尖组织培养获得无病种薯.相继在马铃薯主要产区的许多省份内均取得显著的增产就地留种的效应.但获得的无病毒种薯并不是一劳永逸的,因此必须采用综合措施防止无病毒种薯再侵染病毒和其它病原。例如,应用蚜虫飞迁资料控制传播病毒以及将微型薯和小薯种植在防虫温室和防虫网棚内.在种薯检验过程中应利用酶联免疫和往返凝胶电泳跟踪检测,以便在防虫网棚群内采取局部控制,对脱毒小薯进行无性繁系选择.
China in 1975 has been using potato shoot tip tissue culture to get disease-free seed tubers. Significantly increasing the effect of on-site planting has been achieved in many provinces of the main potato producing areas one after another. However, the virus-free seed tuber obtained is not once and for all, so integrated measures must be taken to prevent the reinfection of virus-free seed tubers with viruses and other pathogens. For example, aphids were used to control the spread of viruses and micro-potatoes and potatoes in insect-proof greenhouses and pest control sheds. Enzyme-linked immunosorbent assay (ELISA) and reciprocation gel electrophoresis (TRIM) should be used in the process of seed potato testing to control local selection of pest free dendrolimus in the pest control net.