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应用ＰＣＲ－ＳＳＣＰ和免疫组化法检测２９例广西南部肝癌（ＨＣＣ）组织中的Ｎ－ｒａｓ基因突变和ＨＢＶ感染状况。结果，肝癌中Ｎ－ｒａｓ基因在第２～３７密码子之间的突变率为７９．３％，其中２２例（７５．８６％）有２～５个突变位点。该基因突变也见于癌旁组织（８０．７７％）。肝组织中ＨＢｓＡｇ和ＨＢｘＡｇ检出率分别为８６．２％和７９．３％，两者具有相关性，并与Ｎ－ｒａｓ基因突变率呈相平行的趋势。因广西南部的肝癌与ＡＦＢ１污染有关，本研究中ＨＣＣ的Ｎ－ｒａｓ基因的突变可能也与ＡＦＢ１的作用有关
PCR-SSCP and immunohistochemistry were used to detect N-ras gene mutation and HBV infection in 29 cases of HCC in southern Guangxi. As a result, the mutation rate of the N-ras gene among the 2nd to 37th codons in HCC was 79.3%, of which 22 cases (75.86%) had 2 to 5 mutation sites. The gene mutation was also found in the adjacent tissue (80.77%). The detection rates of HBsAg and HBxAg in liver tissue were 86.2% and 79.3%, respectively, and they were related to each other, and paralleled the mutation rate of N-ras gene. Since the liver cancer in southern Guangxi is related to AFB1 contamination, the N-ras mutation in HCC may also be related to the role of AFB1 in this study.