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目的 :探讨无机纳米粒子对人肺癌细胞A54 9和小鼠成纤维细胞L92 9生长增殖的影响。方法 :用羟基磷灰石 (HAP)及碳酸钙 (CaCO3 )对A54 9人肺癌细胞和L92 9小鼠成纤维细胞进行体外培养研究 ,分对照组、CaCO3 组和HAP组。绘制细胞生长曲线 ,MTT法测细胞存活率 ,并测集落形成率。结果 :①经CaCO3 纳米粒子处理过的A54 9人肺癌细胞生长曲线明显下降 ,HAP及CaCO3 纳米粒子对L92 9细胞生长曲线无明显影响 ;②CaCO3 纳米粒子在 48h、72h对A54 9细胞的OD值分别为 0 .44± 0 .0 4;0 .42± 0 .0 3,较对照组明显降低 (P <0 .0 1) ;③经CaCO3 纳米粒子处理过的A54 9细胞集落形成率为 33.3% ,较对照组明显降低 (P <0 .0 0 1)。结论 :①CaCO3 对A54 9细胞生长和集落有一定抑制作用 ,对L92 9细胞无明显影响。②HAP对L92 9细胞亦无明显影响。
Objective: To investigate the effect of inorganic nanoparticles on the proliferation and proliferation of human lung cancer cell line A549 and mouse fibroblast L92 9. METHODS: A549 human lung cancer cells and L92 9 mouse fibroblasts were cultured in vitro with hydroxyapatite (HAP) and calcium carbonate (CaCO3) and divided into control group, CaCO3 group and HAP group. The cell growth curve was plotted, the cell survival rate was measured by MTT assay, and the colony formation rate was measured. Results:1 The growth curve of A54 9 lung cancer cells treated with CaCO3 nanoparticles was significantly decreased, and the HAP and CaCO3 nanoparticles had no obvious effect on the growth curve of L92 9 cells; 2 The OD values of CaCO3 nanoparticles on A549 cells at 48 h and 72 h, respectively. It was 0.44±0.04; 0.42±0.30, which was significantly lower than that of the control group (P < 0.01); 3 The colony formation rate of A549 cells treated with CaCO3 nanoparticles was 33.3%. Compared with the control group, it was significantly lower (P < 0.01). Conclusion :1CaCO3 has a certain inhibitory effect on the growth and colony of A549 cells. It has no obvious effect on L92 9 cells. 2HAP also had no significant effect on L92 9 cells.