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本文报道亲脂性氯乙基亚硝脲类4种化合物的气相层析定量法。包括从血浆中用乙醚提取上述化合物。为在无水甲醇中,用碱性催化剂,将原药改变成邻甲基氨基甲酸酯,然后用气相层析分离。用氮敏感检测器或质谱多离子检测器检定。各种方法的最低检出量是每ml血浆100ng。在制备衍生物时,保持本类药物的特性基因,所以亦适用于有生物活性的代谢物测定。试剂:用于转化氯乙基亚硝脲为邻甲基氨基甲酸酯衍生物的试剂,为0.2M的三甲基苯胺氢氧化物无
This paper reports the determination of four compounds of lipophilic chloroethyl nitrosourea by gas chromatography. These include the extraction of the above compounds from plasma with ether. In anhydrous methanol, with a basic catalyst, the original drug was changed to o-methyl carbamate and then separated by gas chromatography. Nitrogen-sensitive detector or mass spectrometry multi-ion detector test. The minimum detectable amount for each method is 100 ng per ml of plasma. In the preparation of derivatives, to maintain the characteristics of the drug gene, it also applies to bioactive metabolite determination. Reagents: Reagents for the conversion of chloroethylnitrosourea to ortho-methylcarbamate derivatives, 0.2 M Trimethylaniline hydroxide None