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目的探讨解偶联蛋白2(UCP-2)基因8号外显子3′-末端45bp插入/缺失多态与糖尿病合并脑血栓的关系。方法178例糖尿病患者(伴有脑血栓组49例,不伴脑血栓组129例),检测所有患者的基因组DNA,用聚合酶链反应(PCR)方法对UCP-2基因进行扩增,记录各组UCP-2等位基因及基因型频率并进行比较。结果UCP-2基因8号外显子3′-末端45bp插入/缺失多态在伴有脑血栓组和不伴脑血栓组中基因型分布差异无统计学意义(χ2=1.17,P>0.05),两组的等位基因频率差异亦无统计学意义(χ2=0.24,P>0.05)。结论UCP-2基因8号外显子3′-末端45bp插入/缺失多态与中国人糖尿病并发脑血栓无相关性。
Objective To investigate the relationship between the 45 bp insertion / deletion polymorphism at the 3’-end of UCP-2 gene and diabetes mellitus complicated with cerebral thrombosis. Methods A total of 178 patients with diabetes mellitus (49 with cerebral thrombosis and 129 without cerebral thrombosis) were enrolled in this study. Genomic DNA was detected in all patients. UCP-2 gene was amplified by polymerase chain reaction (PCR) Group UCP-2 alleles and genotype frequency and compared. Results There was no significant difference in genotype distribution between UCP-2 gene exon 3’-end 45bp insertion / deletion polymorphism and cerebral thrombosis (χ2 = 1.17, P> 0.05) There was no significant difference in allele frequencies between the two groups (χ2 = 0.24, P> 0.05). Conclusion The 45 bp insertion / deletion polymorphism of UCP-2 gene exon 8 at exon 3 has no correlation with cerebral thrombosis in Chinese patients with diabetes mellitus.