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【目的】优化野蔷薇根多糖的过氧化氢(H_2O_2)法脱色工艺条件,为野蔷薇根多糖的脱色纯化提供技术支持。【方法】以野蔷薇根为试验材料,以多糖脱色率和保留率为考察指标,利用响应曲面法对H_2O_2法脱色野蔷薇根多糖的工艺条件进行优化。【结果】通过响应曲面法建立野蔷薇根多糖脱色的回归模型为:Y=94.84+3.08A+1.83B+0.88C+0.17AB+0.23AC-0.055BC-3.17A2-1.31B2-0.87C2(Y为多糖脱色率,A为H_2O_2体积分数、B为溶液p H、C为脱色时间),该模型拟合度较好。H_2O_2法脱色野蔷薇根多糖的最佳工艺条件为:H_2O_2体积分数35.0%、溶液p H 9.00、脱色时间4.5 h,在此条件下多糖脱色率和保留率分别为95.48%和75.17%,脱色率与理论值96.55%的相对误差为1.11%。H_2O_2体积分数、溶液p H和脱色时间3个因素对野蔷薇根多糖脱色效果均有极显著影响(P<0.01)。【结论】H_2O_2脱色法具有操作简便、脱色效果明显、成本低等优点,可对野蔷薇根多糖进行有效脱色。
【Objective】 The objective of this study was to optimize the decolorization conditions of hydrogen peroxide (H_2O_2) in Broussonetia papyrifera and provide technical support for decolorization and purification of Briar. 【Method】 The broussonetia papyrifera was used as test material, and the decolorization rate and retention rate of polysaccharides were taken as the index to evaluate the process conditions of H_2O_2 decolorizing Broussonetia papyrifera polysaccharide by response surface methodology. 【Result】 The regression model of Rosa rugosa root polysaccharide decoloration by response surface methodology was as follows: Y = 94.84 + 3.08A + 1.83B + 0.88C + 0.17AB + 0.23AC-0.055BC-3.17A2-1.31B2-0.87C2 (Y Is the decolorization rate of polysaccharide, A is the volume fraction of H 2 O 2, B is the solution p H, C is the decolorization time). H 2 O 2 decolorization Broussosa root polysaccharide optimum conditions: H 2 O 2 volume fraction 35.0%, p H 9.00 solution, decolorization time 4.5 h, under these conditions, the decolorization rate and retention rate were 95.48% and 75.17%, respectively, the decolorization rate The relative error from the theoretical value of 96.55% is 1.11%. H 2 O 2 volume fraction, solution pH and decolorization time had significant effects on the decolorization of Radix et Rhizoma polysaccharides (P <0.01). 【Conclusion】 The H 2 O 2 decolorization method has the advantages of simple operation, obvious decoloring effect and low cost, and can effectively remove the polysaccharides from the wild rose root.