在液相色谱里,分离取决于所选用的流动相和固定相,即溶剂和色谱柱。总分辨率可表示为:R_s=(1/4)N~(1/2)((α-1)/α)(K′/(K′+1))(i) (ii) (iii)式中:N—塔板数;α—选择系数;K′—容量比。(i)表示柱效;(ii)表示柱子的选择性;(iii)表示柱子的容量系数。由此可见,欲提高分辨率 Rs 有以下三种 In liquid chromatography, the separation depends on the mobile phase and the stationary phase chosen, ie the solvent and the column. The total resolution can be expressed as: R_s = (1/4) N ~ (1/2) ((α-1) / α) (K ’/ (K’ + 1)) (ii) (iii) Where: N-plate number; α-select coefficient; K’-capacity ratio. (i) indicates the efficiency of the column; (ii) indicates the selectivity of the column; and (iii) indicates the capacity coefficient of the column. Thus, to improve the resolution Rs have the following three