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目的 :探讨紫杉醇对成骨肉瘤细胞的杀伤作用及机制。方法 :应用形态学观察 ,MTT分析法 ,DNA梯状电泳 ,流式细胞术检测术 ,研究紫杉醇对成骨肉瘤细胞系SOSP - 96 0 7的细胞毒效应。结果 :紫杉醇作用后成骨肉瘤细胞形态改变明显 ,存活率明显下降。形态学观察 ,DNA梯状电泳 ,流式细胞术检测术证实紫杉醇可诱导成骨肉瘤凋亡。结论 :紫杉醇在体外对成骨肉瘤细胞株SOSP - 96 0 7有较强的杀伤作用 ,并且这种作用主要是通过诱导凋亡实现的
Objective: To investigate the killing effect and mechanism of paclitaxel on osteosarcoma cells. Methods: The cytotoxic effect of paclitaxel on the osteosarcoma cell line SOSP - 96 0 7 was studied by morphological observation, MTT assay, DNA ladder electrophoresis and flow cytometry. Results: Osteosarcoma cell morphology changed obviously after paclitaxel treatment, and the survival rate decreased obviously. Morphological observation, DNA ladder electrophoresis, flow cytometry confirmed that paclitaxel can induce osteosarcoma apoptosis. Conclusion: Paclitaxel has a strong killing effect on osteosarcoma cell line SOSP - 96 0 7 in vitro and this effect is mainly induced by apoptosis