论文部分内容阅读
荧光成像系统的技术指标、采集的荧光图像信噪比和单分子定位算法是影响单分子定位精度的3大因素.在介绍单分子定位的基本极限精度和理论计算精度的基础上,提出了一种被称为图像去噪高斯掩膜算法的单分子定位算法.依据实验用单分子成像系统的具体配置,通过计算机仿真表明,该算法良好的去噪处理和高斯加权质心运算方式,不仅能实现纳米级的定位精度,而且能突破一般理论计算精度的限制,接近基本极限精度,特别是在背景噪声较大的情况下,其优势更加明显.这对单分子的精确定位和跟踪、扩散方式分析、转移速率计算等具有重要的意义.
The technical index of fluorescent imaging system, the acquired signal-to-noise ratio of fluorescent image and the single molecule localization algorithm are the three major factors affecting the single molecule localization accuracy.On the basis of introducing the basic limit precision and theoretical calculation accuracy of single molecule localization, A kind of single-molecule localization algorithm called image denoising Gaussian mask algorithm.According to the specific configuration of experimental single-molecule imaging system, the computer simulation shows that this algorithm is good denoising and Gaussian weighted centroid computing, not only can achieve And it can overcome the limitation of general theoretical calculation accuracy and approach the basic limit precision, especially in the case of large background noise, which is more obvious for the precise positioning and tracking of single molecules and the analysis of diffusion modes , Transfer rate calculation is of great significance.