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目的:从14份标签上注明的来自不同地区的4种干燥蛇粗毒——分别来自舟山眼镜蛇、孟加拉眼镜蛇、尖吻蝮蛇和短尾蝮蛇中提取总DNA,并结合PCR和序列分析技术对粗毒的来源进行核实。方法:分别从上述粗毒中提取总DNA,同时以4种对应蛇肌肉组织中提取的总DNA作为对照,用1对线粒体16SrRNA基因通用引物16H1/16L1进行扩增。结果:测序结果提交NCBI,并与GenBank中的同源序列进行比对和构建进化树。结论:序列分析结果证实扩增的均为正确的来自对应蛇种的线粒体16S基因序列,该技术有可能推广用于对各种动物毒液类毒素样品来源的身份进行核实。
OBJECTIVE: To extract total DNA from four dried snake venoms from different regions, identified on 14 labels, from Zhoushan Cobra, Bengal Cobra, Agkistrodon triceratops and Mackerel snakehead, and PCR and sequence analysis Technology to verify the source of crude poison. Methods: The total DNA was extracted from the above venom. The total DNA extracted from the four corresponding snake muscle tissues was used as a control, and a pair of mitochondrial 16S rRNA gene universal primers 16H1 / 16L1 was amplified. Results: The sequencing results were submitted to NCBI and compared with the homologous sequences in GenBank to construct phylogenetic tree. CONCLUSION: Sequence analysis confirmed that the amplified 16S gene sequences were all correctly derived from the corresponding snake species. This technique may be extended to verify the origin of various animal venom toxoid samples.