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目的探讨内蒙古地区汉族人群类风湿关节炎(RA)与HLA-DRB1基因共同表位(SE)的关联性。方法采用特异性引物聚合酶链反应(PCR-SSP)方法对内蒙古地区汉族人群80例RA患者及110名正常健康者的HLA-DRB1*01、*04、*10的17个等位基因进行检测。结果内蒙古地区RA患者中携带有SE的基因频率显著高于正常对照组(48．8％：20％,P＜0．01),HLA-DR4亚型*0405是主要的易感基因(28．8％：12％,P＜0．01),其他亚型包括DRB1*0101(2．5％：0．9％),*0102(2．5％：0),*0103(1．25％：0．9％),*0104(2．5％：0％),*0401(6．25％：1．8％),*0402(3．75％：0．9％),*0403(1．25％：1．8％),*0404(2．5％：1．8％),*0406(2．5％：2．7％),*0407(1．25％：0．9％),*0408(3．75％：0．9％),*0409(1．25％：0),*0410(2．5％：0．9％),*0411(0：0)和*001(8．75％：4．5％)的差异均无统计学意义。Logistic回归分析表明：SE纯合子对RA的危害性要比杂合子大(P＜0．01)。在RA患者中。SE纯合子和SE杂合子的病程、关节肿痛数、关节压痛数、血沉(ESR),C反应蛋白(CRP)、类风湿因子(RF)、X线(Ⅲ期+Ⅳ期)与SE阴性组比较,差异有统计学意义。SE纯合子与SE杂合子相比较,各观察指标差异均无统计学意义。结论SE与内蒙古地区汉族RA易感性及疾病严重性有关。
Objective To explore the association between rheumatoid arthritis (RA) and HLA-DRB1 gene common epitope (SE) in Han nationality in Inner Mongolia. Methods The HLA-DRB1 * 01, * 04, * 10 17 alleles of 80 RA patients and 110 healthy controls in Han nationality in Inner Mongolia were detected by polymerase chain reaction-specific polymerase chain reaction (PCR-SSP) . Results The frequencies of SE carrying SE gene in RA patients in Inner Mongolia were significantly higher than those in normal controls (48.8% vs 20%, P <0.01). HLA-DR4 subtype * 0405 was the predominant susceptibility gene (28. Other subtypes included DRB1 * 0101 (2.5%: 0.9%), * 0102 (2.5%: 0), * 0103 (1.25% : 0.9%), * 0104 (2.5%: 0%), * 0401 (6.25%: 1.8%), * 0402 (3.75%: 0.9%), * 0403 1.25%: 1.8%), * 0404 (2.5%: 1.8%), * 0406 * 0408 (3.75%: 0.9%), * 0409 (1.25%: 0), * 0410 (2.5%: 0.9%), * 0411 (0: 0) and * 001 (8.75%: 4.5%) showed no significant difference. Logistic regression analysis showed that homozygotes of SE were more harmful to RA than heterozygotes (P <0.01). In RA patients. The course of SE homozygotes and SE heterozygosities, joint swelling and pain, joint tenderness, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF), X-ray Group comparison, the difference was statistically significant. SE homozygous and SE heterozygotes compared to the various indicators were no significant difference. Conclusions SE is associated with RA susceptibility and severity of disease in Inner Mongolia.