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昆虫I家族N-乙酰-D-己糖胺酶Hex1(N-acetyl-D-hexosaminidase family I)是昆虫蜕皮和变态发育中不可或缺的几丁质水解酶.下调或干扰Hex1基因表达水平将导致昆虫蜕皮不正常而死亡.Hex1在生理功能上是特异的表皮几丁质降解酶,功能区别于人类以及高等动植物的同源酶,因此,Hex1可能成为潜在的农药创制靶标.近10年来,昆虫Hex1的研究在生物学和化学生物学等领域取得了突出进展.其中,来源于农业害虫亚洲玉米螟—Hex1(OfHex1)的晶体结构的解析取得重要进展,为针对Hex1的农用小分子理性设计提供理论基础.本文重点针对OfHex1的生理功能、结构特征以及选择性抑制剂等方面的研究进展进行综述.
The insect I family N-acetyl-D-hexosaminidase family 1 (N-acetyl-D-hexosaminidase family I) is an essential chitin hydrolase in insect molting and metamorphosis. Down-regulation or interference with Hex1 gene expression levels Leading to abnormal molting of insects and death.Hex1 is a physiologically unique epidermal chitin-degrading enzyme, which is different from the homologous enzymes in humans and higher plants and animals, therefore, Hex1 may be a potential target for pesticide creation. , The research on insect Hex1 has made outstanding progress in the fields of biology and chemical biology.Among them, significant progress has been made in the analysis of the crystal structure of Ofxylon (HEX1) derived from the agricultural pest, Hex1, Design provides the theoretical basis.This article focuses on the physiological function of OfHex1, structural characteristics and selective inhibitors and other aspects of research are reviewed.