目的:建立血浆中黄酮哌酯活性代谢物MFCA浓度测定的HPLC法。方法:血浆样品经碱化、水解及酸化后,用乙酸乙酯提取。以甲醇-20mmol/LKH2PO4(70∶30)为流动相,紫外310nm检测。结果:线性范围0.25～32μg·ml-1,萃取回收率96.1%～100.3%。加样回收率96.8%～104.0%,日内RSD0.52%～2.30%,日间RSD1.39%～2.97%。结论:本法准确可靠,用于盐酸黄酮哌酯人体药动学研究取得良好结果 OBJECTIVE: To establish a HPLC method for the determination of the active metabolite of flavoxate in plasma. Methods: The plasma samples were alkalized, hydrolyzed and acidified, then extracted with ethyl acetate. Methanol -20mmol / L KH2PO4 (70:30) as the mobile phase, UV detection at 310nm. Results: The linear range of 0.25 ~ 32μg · ml-1, extraction recovery 96.1% ~ 100.3%. The recoveries of samples ranged from 96.8% to 104.0%, RSD from 0.52% to 2.30% and RSD from 1.39% to 2.97% during the day. Conclusion: This method is accurate and reliable, and good results have been obtained in human pharmacokinetics of flavoxate hydrochloride