华支睾吸虫存在于中国、日本、朝鲜等国家,引起宿主的肝胆管疾病。为建立特异性血清学诊断方法,对华支睾吸虫的抗原蛋白进行了分析,从华支睾吸虫成虫中分离、纯化出7kDa蛋白,研究其特性并进行了基因测序,同时采用免疫电镜对其进行了抗原定位。华支睾吸虫特异性蛋白的纯化采用6 Clonorchis sinensis exists in China, Japan, North Korea and other countries, causing the host of hepatobiliary disease. In order to establish a specific serological diagnostic method, the antigenic protein of Clonorchis sinensis was analyzed. The 7 kDa protein was isolated and purified from the adult Clonorchis sinensis, its characteristics were studied and its gene sequencing was carried out. At the same time, its immunoelectron microscopy Antigen localization was performed. Clonorchis sinensis-specific protein purification using 6