用ＡＢＣ法对胆囊炎胆石症患者４１例、胆囊癌患者５例作ＰＣＮＡ测定。发现胆囊癌时ＰＣＮＡ阳性４例（８０％），胆石症时ＰＣＮＡ阳性１８例（４３．９％），两组间有统计学意义（Ｐ＜０．００１）。ＰＣＮＡ阳性中胆石症患者的病吏在５年以上。胆囊癌患者年龄在６０岁以上。提示年龄在６０岁并且病史５年的胆石症患者，需作手术治疗，尤其是女性。 The ABC method was used for the determination of PCNA in 41 cases of gallstone cholelithiasis and 5 cases of gallbladder cancer. PCNA positive was found in 4 cases (80%) when gallbladder cancer was detected, and 18 cases (43.9%) were positive in PCNA when gallstone disease was present. There was a statistically significant difference between the two groups (P<0.001). The disease in patients with PCNA positive gallstone disease is more than 5 years. Gallbladder cancer patients are over 60 years old. Patients with gallstone disease, who are 60 years old and have a history of 5 years, need surgical treatment, especially women.