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目的研究脑忆源片对大鼠脑组织中单胺类递质含量的影响,为探讨其改善学习记忆作用的机制提供理论依据。方法大鼠连续给药15 d后取样,采用HPLC-ECD法检测空白组和给药组大鼠脑组织中单胺类神经递质的含量;色谱柱:Phenomenex luna C18(150×4.60 mm,5μm);流动相:200 mmol.L-1磷酸二氢钠水溶液-乙腈(87∶13);流速:1 ml.min-1;柱温:35℃。结果 NE、E、DA、5-HIAA及5-HT五种物质在0.0095～10.5 ng.μL-1之间线性关系均良好(r≥0.9996);E的最低检测限低于5 ng.mL-1,NE、DA、5-HIAA、5-HT的最低检测限均低于1.21 ng.mL-1,五种物质平均回收率均在84.41%～100.92%之间。与空白组相比,高、中、低剂量组NE、E、DA含量均升高,5-HT含量均降低,且NE、DA及5-HT含量变化与剂量呈量效关系。结论该方法具有准确、灵敏、简便等优点,可用于大鼠脑组织中单胺类神经递质的检测;脑忆源片能明显提高大鼠脑组织中NE、DA等易化记忆过程递质的含量,同时,又降低了对记忆有阻抑作用的5-HT的含量,提示脑忆源片辅助改善学习记忆的作用可能与其改变脑组织内单胺类递质的含量有关。
Objective To study the effect of Nanyuyuan Tablets on monoamine neurotransmitters in rat brain and to provide theoretical basis for exploring its mechanism of improving learning and memory. Methods The rats were sampled 15 days after continuous administration. The concentrations of monoamine neurotransmitters in the brain tissue of the blank group and the treated group were measured by HPLC-ECD. The chromatographic column was Phenomenex luna C18 (150 × 4.60 mm, 5μm ); Mobile phase: 200 mmol.L-1 sodium dihydrogen phosphate aqueous solution-acetonitrile (87:13); flow rate: 1 ml.min-1; Results The linear relationship between NE, E, DA, 5-HIAA and 5-HT was between 0.0095 and 10.5 ng · μL-1 (r≥0.9996). The minimum detection limit of E was lower than 5 ng · mL- 1, the detection limits of NE, DA, 5-HIAA and 5-HT were all lower than 1.21 ng.mL-1. The average recoveries of five substances ranged from 84.41% to 100.92%. Compared with the blank group, NE, E and DA contents in high, medium and low dose groups were increased, while 5-HT content was decreased, and the dose-effect relationship between NE, DA and 5-HT content was dose-dependent. Conclusion The method is accurate, sensitive and simple. It can be used for the detection of monoamine neurotransmitters in brain tissue of rats. Nao Yi Yuan Tablet can obviously improve the neurotransmitter contents of NE, DA and so on , While reducing the inhibitory effect on memory of 5-HT content, suggesting that Meixian tablets to help improve the learning and memory may be related to changes in the contents of monoamine neurotransmitters in the brain tissue content.