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[目的]探讨TTF1对肿瘤血管生成的抑制作用.[方法]建立人肝癌HepG-2细胞裸鼠移植瘤模型,取40只随机分为模型对照组、芹菜素组、小、中、大剂量(5,10,20μmol/g)TTF1组,药物干预21d后处死裸鼠;采用免疫组织化学方法检测CD34,以Weidner微血管计数法计算肿瘤微血管密度(MVD);应用Western-blot方法检测血管内皮生长因子(VEGF)、成纤维细胞生长因子(bFGF)、环氧合酶-2(COX-2)、缺氧诱导因子-1α(HIF-1α)和KDR.[结果]大、中、小剂量TTF1组MVD明显下降,肿瘤组织VEGF,KDR,bFGF,HIF-1α和COX-2蛋白的表达明显下调.[结论]TTF1对肿瘤血管生成有明显的抑制作用,其机制可能与下调VEGF,KDR,bFGF,HIF-1α和COX-2蛋白有关联.
[Objective] To investigate the inhibitory effect of TTF1 on tumor angiogenesis. [Methods] To establish a model of HepG-2 human hepatoma xenografts in nude mice. Forty randomly divided models were divided into model control group, apigenin group, small, medium and large dose 5, 10, 20μmol / g) TTF1 group. The mice were sacrificed 21 days after the drug intervention. CD34 was detected by immunohistochemistry and the microvessel density (MVD) was calculated by Weidner microvascular count method. The expressions of vascular endothelial growth factor (VEGF), bFGF, COX-2, HIF-1α and KDR were measured by ELISA. [Results] The expression of VEGF, KDR, bFGF, HIF-1α and COX-2 in tumor tissues was significantly decreased. [Conclusion] TTF1 can significantly inhibit tumor angiogenesis, which may be related to the downregulation of VEGF, KDR, bFGF, HIF-1α is associated with COX-2 protein.