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研究蓝舌病毒中国湖北分离株(BTVHbC株)在Vero和BHK21两种细胞上的增殖特点以及与标准病毒株(BTV10)的血清学关系。发现BTVHbC和BTV10感染Vero和BHK21单层细胞后,24~30h病毒滴度可达到最高值。用BTVHbC免疫家兔,其抗血清在琼脂双扩试验中能与BTV10抗原病毒呈强阳性反应而与轮状病毒、风疹病毒、柯萨奇病毒、流感病毒等呈阴性反应。
To investigate the proliferation of BTVHbC strain in two cell lines of Vero and BHK21 and the serological relationship with BTV10 strain. Found BTV HbC and BTV 10 infected Vero and BHK 21 monolayers, 24 ~ 30h virus titer reached the highest value. Rabbits were immunized with BTVHbC and their antisera reacted strongly with BTV10 antigen in agar doublewell assay and negative with rotavirus, rubella virus, coxsackievirus and influenza virus.