论文部分内容阅读
为建立丙型肝炎病毒 (HCV )体外感染和细胞培养系统 ,用定量的 HCV RNA阳性血清感染人肝癌细胞系 (Hep G2细胞系 ) ,应用地高辛标记 HCV RNA探针原位杂交技术和 RT- PCR方法对感染后的细胞和上清液中的 HCV RNA进行了检测。在感染后的第一代至第七代的细胞中出现特异性杂交阳性信号 ,第一代、第二代和第六代检测出 HCV RNA正链 ,并在感染后第一、二代检测出 HCV RNA负链。显示 HCV不仅能在体外感染 Hep G2细胞系 ,而且有基因的复制 ,证明 Hep G2细胞能作为 HCV的体外细胞培养系。
To establish a Hepatitis C virus (HCV) in vitro infection and cell culture system, human hepatocellular carcinoma cell lines (Hep G2 cell line) were infected with a quantitative HCV RNA positive sera, labeled with digoxigenin labeled HCV RNA probe in situ hybridization and RT - The HCV RNA in the infected cells and supernatant was detected by PCR. Specific hybridization positive signals appeared in the first to the seventh generation of infected cells. The first, the second and the sixth generation detected positive HCV RNA, and the first and the second generation detected after infection Negative strand of HCV RNA. It is shown that HCV can infect Hep G2 cell line in vitro and have gene duplication, which proves that Hep G2 cell can serve as an in vitro cell culture line of HCV.