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报道了农抗5102产生菌同源菌株90-11、FR-008及WH-1相互之间融合研究的前期结果。3菌株原生质体制备的适宜酶解温度和时间均为32℃和1.0~1.5h,但所需溶菌酶量则有异:90-11为2~4mg/ml,FR-008为6mg/ml,WH-1为2mg/ml。将各菌株的原生质体在-20℃下冻存1个月内的相对再生率可维持在70%以上,2个月以内仍可达60%以上。以50%PEG1000诱导融合各组合的融合频率是,90-11×WH-1为10-2,FR-008×-90-11以及FR-008×WH-1均为10-3。
The preliminary results of the study on the fusion of strains of Nongkang 5102 with homologous strains 90-11, FR-008 and WH-1 were reported. 3 strain protoplast preparation of the appropriate temperature and time for the hydrolysis are 32 ℃ and 1.0 ~ 1.5h, but the required amount of lysozyme is different: 90-11 2 ~ 4mg / ml, FR-008 is 6mg / Ml, WH-1 is 2 mg / ml. The relative regeneration rate of the protoplasts of each strain at -20 ° C within 1 month can be maintained at over 70%, and still up to 60% within 2 months. The frequency of fusion for each combination induced by 50% PEG 1000 was 90-2, WH-1 was 10-2, FR-008 × -90-11 and FR-008 × WH-1 were all 10-3.