Acute and subacute oral toxicity study on the flavonoid rich fraction of Monodora tenuifolia seed in

来源 :Asian Pacific Journal of Tropical Biomedicine | 被引量 : 0次 | 上传用户:yangmingli1213
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Objective:To investigate the effects of the flavonoid rich fraction of Monodora tenuifolia seed on the haematology,histopathology and liver profile of Wistar albino rats.Methods:Toxicity study was investigated on the flavonoid rich fraction of Monodora tenuifolia in rats administered different concentrations orally for 28 d using standard laboratory procedures.Results:The LD_(50)of the flavonoid rich fraction was found to be above 5000 mg/kg body weight in mice observed for 48 h.After the Day 14,biochemical markers of liver injury such as serum alanine aminotransferase,and aspartate aminotransferase decreased significantly(P<0.05 at doses of 100 and 200 mg/kg body weight and P<0.01 at 400 mg/kg)while serum alkaline phosphatase increased non-significantly(P>0.05).There was non-significant(P>0.05)effect observed across the groups in the levels of serum total protein,albumin,globulin,urea and creatinine.The result of histological examination showed various degrees of peribiliary hepatitis after the Day 14 which fizzled out after the Day 28.Conclusions:The result therefore suggests that the seed extract is potentially safe. Objective: To investigate the effects of the flavonoid rich fraction of Monodora tenuifolia seed on the haematology, histopathology and liver profile of Wistar albino rats. Methods: Toxicity study was investigated on the flavonoid rich fraction of Monodora tenuifolia in rats administering different concentrations orally for 28 d using standard laboratory procedures. Results: The LD_ (50) of the flavonoid rich fraction was found to be above 5000 mg / kg body weight in mice observed for 48 h. After the Day 14, biochemical markers of liver injury such as serum alanine aminotransferase, and aspartate aminotransferase decreased significantly (P <0.05 at doses of 100 and 200 mg / kg body weight and P <0.01 at 400 mg / kg) while serum alkaline phosphatase increased non-significantly significant (P> 0.05) effect observed across the groups in the levels of serum total protein, albumin, globulin, urea and creatinine. The result of histological examination showed various degrees of peribiliary hepatitis after the Day 14 which fizzled out after the Day 28.Conclusions: The result causes suggests that the seed extract is potentially safe.
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