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研究黄芪、灵芝、茯苓、党参、当归、天花粉等中药多糖对红细胞膜相CD35免疫活性的调节作用。方法 :采用 10 %CD35单克隆抗体阻断试验 ,比较以上中药多糖在 10 %CD35单克隆抗体阻断前后红细胞膜相CD35活性的变化。结果 :4 0例健康献血员和 30例恶性肿瘤患者的红细胞经以上中药多糖作用后 ,其RBC .C3bRR与对照组相比明显提高 (P <0 .0 1)。用 10 %CD35单克隆抗体阻断恶性肿瘤患者的红细胞膜相CD35后 ,其RBC .C3bRR明显下降 (P <0 .0 1)。用以上中药多糖作用过的补体致敏酵母菌作红细胞RBC .3bRR试验 ,其花环率无明显变化 (P >0 .0 5 ) ,而将以上中药多糖作用过的红细胞作红细胞RBC .C3bRR试验 ,其花环率明显升高 (P <0 .0 1)。结论 :研究说明以上中药多糖能增强健康献血员和恶性肿瘤患者的红细胞免疫功能 ,并提示以上中药多糖调节红细胞膜相CD35免疫活性的机制 ,与补体致敏酵母菌无关 ,而与红细胞膜相CD35活性有关。
To study the regulatory effects of Radix Astragali, Ganoderma lucidum, Poria cocos, Codonopsis pilosula, Angelica sinensis, and TCS on the immune activity of red cell membrane CD35. METHODS: The 10% CD35 monoclonal antibody blocking assay was used to compare the changes of CD35 activity of red blood cell membranes before and after the blockage of 10% CD35 monoclonal antibody. Results : The RBC.C3bRR of 40 healthy blood donors and 30 patients with malignant tumors were significantly higher than those of the control group (P < 0.01). After blocking the red blood cell membrane CD35 of malignant tumor patients with 10% CD35 monoclonal antibody, the RBC.C3bRR was significantly decreased (P < 0.01). The red blood cell RBC .3bRR test was performed on the sensitized yeasts that had been treated with the traditional Chinese medicine polysaccharides, and the rosette rate had no significant change (P > 0.05). The red blood cells treated with the polysaccharides of the Chinese traditional medicines were used as RBC.C3bRR test. The rosette rate was significantly higher (P < 0.01). Conclusion: The study shows that the traditional Chinese medicine polysaccharides can enhance the red blood cell immune function of healthy blood donors and patients with malignant tumors, and suggest that the traditional Chinese medicine polysaccharides regulate the mechanism of the red blood cell membrane CD35 immune activity, and has nothing to do with the complement-sensitized yeast, but with the red blood cell membrane CD35 Activity related.