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目的:制作食积肺炎动物模型,探讨银莱汤对动物模型肠黏膜机械屏障的作用机制,证明肺与胃肠同治理论的有效性,为指导临床疾病的防治提供实验研究依据。方法:实验小鼠随机分为正常组、感染组、食积组、食积感染组、食积治疗组、食积感染治疗组。制作食积肺炎动物模型,给予银莱汤治疗,观察实验各组肠黏膜形态学变化、肠组织ZO-1及血清内毒素水平。结果:食积组、食积感染组小鼠肠黏膜上皮细胞及杯状细胞增大增多,排列紊乱;食积治疗组、食积感染治疗组肠黏膜上皮细胞及杯状细胞排列整齐。食积组、食积感染组小鼠肠组织ZO-1水平较正常组显著下降(P<0.01);食积治疗组显著高于食积组(P<0.01);食积感染治疗组显著高于食积感染组(P<0.01)。各组小鼠血清内毒素水平与正常组比较均无显著性差异。结论:肺与胃肠积热会影响肠黏膜分泌功能,造成肠黏膜机械屏障损伤,其损伤机制与肠黏膜ZO-1的破环有关。银莱汤能够调节肠黏膜分泌功能,修复肠黏膜ZO-1,对肠黏膜机械屏障具有保护作用。
OBJECTIVE: To make animal models of food-borne pneumonia, investigate the mechanism of Yin Lai Tang on the animal model of intestinal mucosal mechanical barrier, and to prove the effectiveness of the theory of lung and gastrointestinal intestine to provide experimental evidence for the prevention and treatment of clinical diseases. Methods: The experimental mice were randomly divided into normal group, infection group, food product group, food accumulation group, food accumulation group and food accumulation group. Food pneumonia model was made and treated with Yin Lai Tang. The morphological changes of intestinal mucosa, the intestinal tissue ZO-1 and the level of serum endotoxin were observed. Results: The intestinal mucosa epithelial cells and goblet cells in food plot group and food plot infection group were increased and disordered. The intestinal mucosa epithelial cells and goblet cells in food-treated group and nectar-infected group were arranged neatly. The ZO-1 level in the intestine of mice in the food-accumulation group and the food-accumulation group was significantly lower than that in the normal group (P <0.01), that of the food group was significantly higher than that of the food group (P <0.01) P <0.01). Serum endotoxin levels in all groups showed no significant difference compared with normal group. Conclusion: Accumulation of heat in the lung and gastrointestinal tract may affect the secretion of intestinal mucosa, resulting in mechanical barrier damage of intestinal mucosa. The damage mechanism is related to the destruction of intestinal mucosa ZO-1. Yin Lai soup can regulate intestinal mucosal secretion, repair intestinal mucosa ZO-1, protective effect on the intestinal mucosal mechanical barrier.