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目的:探讨上皮性卵巢癌组织中雌激素受体β(ERβ)与磷酸化细胞外信号调控蛋白激酶1、2(p-ERK1/2)的表达及其与临床病理参数的关系。方法:选取2010年10月至2011年12月青岛大学医学院附属医院经病理证实的上皮性卵巢癌组织标本70例,卵巢良性肿瘤24例,正常卵巢组织24例。采用RT-PCR和免疫组织化学SP法检测上述组织中ERβ和p-ERK1/2的表达。结果:(1)卵巢癌组织中ERβmRNA相对表达水平(0.3764±0.9826)显著低于卵巢良性肿瘤(0.4900±0.3742)及正常卵巢组织(0.4980±0.0434)(P<0.05)。卵巢癌组织中ERβ表达与组织学分型、细胞学分级及淋巴转移有关(P<0.05);ERβ蛋白阳性表达率及其与临床病理特征关系与ERβmRNA一致;(2)ERK1/2 mRNA相对表达水平(0.6007±0.1554、0.6951±1.3694)显著高于卵巢良性肿瘤(0.3575±0.0479、0.5125±0.0411)及正常卵巢组织(0.3027±0.1024、0.5431±0.0811)(P<0.05);ERK1/2表达与细胞学分级、临床分期及腹水有关(P<0.05);p-ERK1/2蛋白阳性表达率及其与临床病理特征与p-ERK1/2 mRNA一致。ERβ蛋白与p-ERK1/2蛋白表达呈负相关(r=-0.282,P<0.05)。结论:ERβ低表达和p-ERK1/2高表达于卵巢癌组织,可能在卵巢癌的发生、发展中有重要作用。
Objective: To investigate the expression of estrogen receptor β (ERβ) and phosphorylated extracellular signal-regulated protein kinase 1,2 (p-ERK1 / 2) in epithelial ovarian cancer and its relationship with clinicopathological parameters. Methods: From October 2010 to December 2011, 70 cases of histologically confirmed epithelial ovarian cancer specimens from Affiliated Hospital of Medical College of Qingdao University, 24 cases of benign ovarian tumor and 24 cases of normal ovarian tissue were selected. The expression of ERβ and p-ERK1 / 2 in these tissues was detected by RT-PCR and immunohistochemistry. Results: (1) The relative expression level of ERβ mRNA in ovarian cancer tissues (0.3764 ± 0.9826) was significantly lower than that in benign ovarian tumors (0.4900 ± 0.3742) and normal ovarian tissue (0.4980 ± 0.0434) (P <0.05). The expression of ERβ was correlated with the histological type, cytological grade and lymphatic metastasis in ovarian cancer (P <0.05). The positive expression rate of ERβ protein was correlated with the clinicopathological features of ERβmRNA. (2) The relative expression level of ERK1 / 2 mRNA (0.6007 ± 0.1554,0.6951 ± 1.3694) were significantly higher than those in benign ovarian tumors (0.3575 ± 0.0479,0.5125 ± 0.0411) and normal ovarian tissues (0.3027 ± 0.1024,0.5431 ± 0.0811) (P <0.05). The expressions of ERK1 / 2 and cytokines (P <0.05). The positive rate of p-ERK1 / 2 protein and its clinicopathological characteristics were consistent with those of p-ERK1 / 2 mRNA. ERβ protein and p-ERK1 / 2 protein expression was negatively correlated (r = -0.282, P <0.05). Conclusion: The low expression of ERβ and high expression of p-ERK1 / 2 in ovarian cancer may play an important role in the development and progression of ovarian cancer.