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目的评估异基因造血干细胞移植后Ｔ淋巴细胞免疫功能状态。方法采用三色荧光标记的流式细胞术，对４例异基因造血干细胞移植、２例自体干细胞移植患者移植前后的Ｔ淋巴细胞及其亚群的ＦａｓＬ表达进行监测。结果移植前Ｔ细胞的ＴａｓＬ、表达率仅（２.１３％±２.０２％），与正常对照组无显著差异（Ｐ＞０.０５），移植后异基因造血干细胞移植患者ＦａｓＬ表达水平升高，尤以急性ＧＶＨＤ患者表达升高显著（Ｐ<０.０５），而自体干细胞移植患者的ＦａｓＬ表达未见明显改变。结论异基因造血干细胞移植术后部分Ｔ淋巴细胞处于激活状态； ＦａｓＬ表达可能与ａＧＶＨＤ相关；对ＦａｓＬ表达的监测可从细胞、免疫分子两个层次对Ｔ细胞免疫功能状态进行评估，该体系的完善对临床判断预后、指导治疗可能有较直接的参考价值。
Objective To evaluate the immune function of T lymphocytes after allogeneic hematopoietic stem cell transplantation. Methods The expression of FasL in T lymphocytes and their subpopulations before and after transplantation in 4 patients with allogeneic hematopoietic stem cell transplantation and 2 patients with autologous stem cell transplantation was monitored by three-color fluorescence-labeled flow cytometry. Results The expression of TasL in pretransplant T cells was only (2.13% ± 2.02%), which was not significantly different from that in normal controls (P> 0.05). The level of FasL expression in allogeneic hematopoietic stem cell transplantation Especially in patients with acute GVHD (P <0.05), but there was no significant change in FasL expression in autologous stem cell transplantation patients. Conclusions Some T lymphocytes are activated after allogeneic hematopoietic stem cell transplantation. FasL expression may be related to aGVHD. The monitoring of FasL expression can assess the immune function of T cells from two levels of cells and immune molecules. The improvement of this system Prognosis of clinical judgment, guidance and treatment may have a more direct reference value.