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本研究评价了某些蒽醌衍生物和蒽酮的抗人体巨细胞病毒(HCMV)的作用和其细胞毒性,以二羟基丙氧甲基鸟嘌呤DHPG为对照。所有被评价的化合物,除蒽酮大黄素、双蒽酮大黄素及原金丝桃素由作者单位自行合成,并精制使纯度达95％以上外,其余都购自Aldrich试剂公司,不经精制直接使用。所用细胞为人体包皮纤维细胞(HS-68)和人肺纤维细胞(MRC-5),均由美国标准菌库(ATCC)供应并保存在Dulbecco改进的Eagle培养基(DMEM)或基本的Eagle(BME)培养基中。每种培养基都加10％胎牛
This study evaluated the effects of certain quinone derivatives and fluorenone against human cytomegalovirus (HCMV) and its cytotoxicity, using dihydroxypropoxymethylguanine DHPG as a control. All the compounds evaluated, except for fluorenone emodin, diterpene emodin, and proto-hypertonin, were synthesized by the authors and refined to a purity of more than 95%. The rest were all purchased from Aldrich Reagents, without purification. Use directly. The cells used were human foreskin fibroblasts (HS-68) and human lung fibroblasts (MRC-5), both supplied by the American Standard Library (ATCC) and kept in Dulbecco’s modified Eagle’s medium (DMEM) or basic Eagle ( BME) Medium. 10% fetal calf per medium