论文部分内容阅读
短链脂肪酸(SCFAs)是由纤维素性饮食在瘤胃内发酵产生的乙酸、丙酸、丁酸以及戊酸等组成的混合物。SCFA对维持反刍动物的能量平衡至关重要,目前对SCFA的吸收机制和影响因素至今尚不清楚,本试验主要研究一元羧酸转运蛋白(monocarboxylate/proton cotransporter isoform 1,MCT1)在犊牛消化道内的分布及SCFA对MCT1表达的影响。运用免疫印迹Western blot和实时荧光定量PCR检测MCT1在犊牛消化道内的分布和表达;在体外试验中,体外添加一定比例的SCFA(乙酸、丙酸和丁酸的混合物)处理犊牛原代瘤胃上皮细胞,检测SCFA对MCT1表达水平的影响。结果显示:MCT1在整个消化道内均有分布,并且在瘤胃内的表达水平显著高于其他部位(P<0.01);体外处理的瘤胃上皮细胞,正常组MCT1的表达水平显著高于(P<0.05)SARA组。结果表明:正常水平的SCFA能够促进MCT1的表达和转运能力,而过量的SCFA抑制MCT1的表达和转运能力。
Short chain fatty acids (SCFAs) are mixtures of acetic acid, propionic acid, butyric acid, and valeric acid, which are produced by fermentation of the cellulite diet in the rumen. SCFA is very important to maintain the energy balance of ruminants. At present, the mechanism of SCFA absorption and its influencing factors remain unclear. In this study, monocarboxylate / proton cotransporter isoform 1 (MCT1) was studied in calf digestive tract Distribution and SCFA on the expression of MCT1. The distribution and expression of MCT1 in calf digestive tract were detected by Western blot and real-time fluorescence quantitative PCR. In vitro experiments, a certain proportion of SCFA (mixture of acetic acid, propionic acid and butyric acid) was added in vitro to treat calf primary rumen Epithelial cells, the detection of SCFA on MCT1 expression levels. The results showed that MCT1 was distributed in the whole digestive tract and the expression level of MCT1 in the rumen was significantly higher than that in other sites (P <0.01). The MCT1 expression in the rumen epithelial cells was significantly higher than that in the normal group (P <0.05) SARA group. The results showed that normal levels of SCFA promoted the expression and transport ability of MCT1, and excessive SCFA inhibited the expression and transport ability of MCT1.