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土壤微生物是土壤中重要的活性组成部分,研究土壤微生物代谢活性对于理解农业措施对农业生态系统的影响至关重要。已有研究表明,将BIOLOG与微量热耦合可揭示中性及碱性土壤中影响代谢活性的碳源因子,但对于酸性土壤鲜有报道。选取一种典型的酸性土壤——红壤为研究对象,采用BIOLOG及微量热技术研究施肥后红壤微生物的碳源底物利用能力及热动力学变化情况,进一步耦合两种技术探讨提高红壤微生物活性的碳源因子。结果表明,有机无机肥配施能显著提高红壤微生物的碳源底物利用能力,改变对碳源的偏好,增强其热代谢活性;但某些引起有机无机肥配施处理分异的碳源未对红壤微生物的热代谢活性产生促进作用,相反产生抑制效果,说明BIOLOG具有一定局限性;L-精氨酸和糖原提高红壤微生物的热代谢活性,其原因在于这两种碳源可能提高红壤中氮、磷循环相关的微生物活性。因此,在红壤中施用富含该类碳源的有机肥对提高红壤微生物活性、提升红壤地力具有积极意义。
Soil microorganisms are important active components in soils. Studying the metabolic activity of soil microorganisms is crucial to understand the impact of agricultural practices on agricultural ecosystems. It has been shown that coupling BIOLOG with trace amounts of heat can reveal carbon sources that affect metabolic activity in neutral and alkaline soils, but few reports have been made on acidic soils. A typical acidic soils, red soil, was selected as research object. The BIOLOG and microcalorimetry were used to study the utilization of carbon substrate and thermodynamic changes of red soil microorganisms after fertilization. Coupled with the two techniques, the soil microbial activity Carbon source factor. The results showed that the combination of organic and inorganic fertilizers could significantly increase the utilization of carbon substrate of red soil microorganisms, change the preference of carbon sources and enhance their thermal metabolic activity; However, some of the carbon sources that caused the differentiation of organic-inorganic fertilizers On the other hand, BIOLOG has certain limitation. L-arginine and glycogen increase the thermo-metabolic activity of microorganisms in red soil. The reason is that the two kinds of carbon sources may increase red soil Medium nitrogen, phosphorus cycle related microbial activity. Therefore, the application of organic fertilizers rich in these carbon sources in red soils is of great significance to improve the activity of microorganisms and soil fertility of red soils.