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目的 建立一个准确易行的诊断非何杰金氏淋巴瘤的实验室指标。方法 建立了以 BCL 2酵母人工染色体克隆 y A15 3A6、以及免疫球蛋白重链基因 (Ig H)噬菌体克隆 H2 4为探针的双标记荧光原位杂交技术 ,用以在间期核中检测 BCL 2原癌基因易位。结果 在滤泡性淋巴瘤细胞系 SU- DHL - 6细胞中 ,BCL 2和 Ig H的杂交信号呈非随机分布 ,与一个 BCL 2等位基因易位到 14号染色体 Ig H基因之上相对应 ,一个 BCL 2和一个 Ig H杂交信号始终紧密相连。而在正常淋巴细胞中 ,这两种基因的杂交信号至少保持 1/ 10核直径的距离。结论 该方法客观明了 ,准确易行 ,不受标本中分裂相数量和质量的限制 ,可望成为临床诊断非何杰金氏淋巴瘤的常规手段。
Objective To establish an accurate and easy laboratory index for the diagnosis of non-Hodgkin’s lymphoma. Methods A dual-labeled fluorescence in situ hybridization was developed based on BCL 2 yeast artificial chromosome clone y A15 3A6 and immunoglobulin heavy chain gene (HH) phage clone H2 4 as a probe to detect BCL 2 protooncogene translocation. Results In the follicular lymphoma cell line SU-DHL - 6, the hybridization signals of BCL 2 and Ig H were nonrandomly distributed, corresponding to the translocation of one BCL 2 allele to the Ig H gene on chromosome 14 , One BCL 2 and one Ig H hybrid are always tightly linked. In normal lymphocytes, the hybridization signals of these two genes maintain a distance of at least 1/10 of the nuclear diameter. Conclusion The method is objective, clear and accurate, and is not limited by the quantity and quality of the split phase in the specimen. It is expected to become a routine method for the clinical diagnosis of non-Hodgkin’s lymphoma.