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目的 :探讨补肾复方用于破骨细胞药效观察的含药血清制备条件之一 (量效关系 )。方法 :取体重 2 70± 2 0 g的 SD大鼠 ,雌雄各半 ,每组 10只 ,同等条件下制备补肾复方含药血清和对照血清 ,分别观察不同灌胃剂量和血清添加量的含药血清对骨吸收陷窝数量的影响。结果 :随着灌胃剂量和含药血清添加量的提高 ,其骨吸收陷窝数明显减少。结论 :补肾复方含药血清对破骨细胞骨吸收功能具有明显的抑制作用 ;补肾复方用于破骨细胞药效观察的大鼠含药血清制备条件之一为等效剂量连续 2次 (间隔 2 h)灌胃、末次灌胃后 1h采血 ,其血清添加浓度为 30 %。
Objective :To investigate the preparation condition of drug-containing serum for the observation of the effect of Bushen compound on osteoclasts (dose-effect relationship). METHODS: Sprague-Dawley rats weighing 2 70±20 g were divided into two groups, 10 females and 10 males. The serum containing the kidney and compound drugs and the control serum were prepared under the same conditions. The medicines with different gavage doses and serum additions were observed. The effect of serum on the number of bone resorption lacunae. RESULTS: With the increase of gavage dose and drug-containing serum, the number of bone resorption lacunae was significantly reduced. Conclusion: The serum containing Bushenfufang has obvious inhibitory effect on the bone resorption of osteoclasts. One of the preparation conditions of Bushenfufang used for the observation of the drug efficacy of rat osteoclasts is the equivalent dosage of 2 consecutive times (2 intervals). h) Gavage, blood sampling 1 h after the last gavage, serum concentration was 30%.