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目的研究中国广东汉族人群中血清、脑脊液(cerebrospinalfluid,CSF)肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)水平及其-308G/A基因多态性与多发性硬化之间的相关性。方法采用双抗体夹心ABC-ELISA法测定68例无亲缘关系的急性发作期多发性硬化(multiplesclerosis,MS)患者和55例非免疫系统疾病患者的血清、CSF的TNF-α水平,同时应用聚合酶链反应-限制性片段长度多态性技术检测上述68例MS患者和106名无血缘关系的广东籍健康汉族人的TNF-α-308G/A基因型。结果发作期MS患者血清中TNF-α水平与非免疫系统疾病患者组间差异有统计学意义(P<0·05),分别是(276±71)pg/mL和(234±76)pg/mL;发作期MS患者CSF中TNF-α水平与非免疫系统疾病患者组差异无统计学意义(P>0·05),分别是(265±78)pg/mL和(245±83)pg/mL;TNF-α-308G/A各等位基因型频率在MS组和正常人组比较差异无统计学意义(P>0·05),MS组TNF-α基因AA基因型和A等位基因频率分别为4.4%和14·0%,正常人对照组分别为0和8.5%。结论(1)发作期MS与血清中TNF-α水平相关,与CSF中TNF-α水平不相关。(2)从目前调查的例数中,中国广东汉族人群TNF-α基因-308G/A多态性与广东人群中MS不相关。
Objective To investigate the correlation between the level of tumor necrosis factor-α (TNF-α) and the polymorphism of -308G / A in serum and cerebrospinal fluid (CSF) and multiple sclerosis in Han nationality in Guangdong Province of China. Methods Serum and CSF TNF-α levels were measured in 68 unrelated patients with acute exacerbation of multiplesclerosis (MS) and 55 non-immune system diseases by double antibody sandwich ABC-ELISA. Polymerase chain reaction The genotypes of TNF-α-308G / A in the above 68 cases of MS patients and 106 unrelated Guangdong healthy Han were detected by PCR and restriction fragment length polymorphism. Results The levels of TNF-α in sera of patients with MS at onset stage were significantly different from those of non-immune system patients (P <0.05), which were (276 ± 71) pg / mL and (234 ± 76) pg / mL. The levels of TNF-α in the CSF of patients with MS at the onset stage were not significantly different from those of the non-immune system patients (P> 0.05), and were (265 ± 78) pg / mL and (245 ± 83) pg / mL; TNF-α-308G / A allele frequencies in MS group and normal group were no significant difference (P> 0.05), MS genotype TNF-α gene AA genotype and A allele The frequencies were 4.4% and 14.0% respectively, while those in the control group were 0 and 8.5% respectively. Conclusions (1) MS in seizure stage is related to the level of TNF-α in serum and not to the level of TNF-α in CSF. (2) From the current number of cases investigated, TNF-α gene-308G / A polymorphism in Guangdong Han population was not related to MS in Guangdong population.