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应用PCR-SSP(PCRamplificationwithsequencespecificprimer)方法将HLAⅡ类DRB1位点基因分型应用于亲权鉴定。对42例亲子鉴定案例进行分析研究的结果表明,本方法简单、快速、结果可靠,且具有较高的非父排除概率(66.3%),不仅适用于法医学亲手鉴定和个人识别,亦可应用于移植配型、HLA相关疾病及人类遗传学研究。
Application of PCR-SSP (PCRamplificationwithsequencespecificprimer) method HLA class DRB1 locus genotyping applied to parental identification. Analysis of 42 cases of paternity test results show that the method is simple, rapid, reliable, and has a high probability of non-parent exclusion (66.3%), not only for forensic identification and personal identification can also be Applied to transplantation matching, HLA-related diseases and human genetics.