采用桶栽方式,对抗旱性强的F172和抗旱性弱的YL6甘蔗品种在苗期进行重度干旱胁迫处理后,应用蛋白质双向电泳技术进行差异蛋白质分析,分别找出差异显著的28和20个差异蛋白点,其中部分呈现上调表达,部分呈现下调表达,还有部分新增的蛋白点,因品种抗性不同而表现各异,F172叶片中的差异蛋白主要表现为上调表达,而YL6中大多表现为下调表达。在重度干旱胁迫下,抗旱性不同的甘蔗品种蛋白质丰度变化有显著差异。采用MALDI-TOF-TOF/MS鉴定所获得的差异蛋白,从YL6、F172中分别鉴定出18、14个蛋白的氨基酸序列,对所鉴定的蛋白质根据功能分为8类。YL6中参与自由基清除的2个,参与光合作用的6个,参与细胞生长和分裂的1个,参与基础代谢的6个,参与防卫反应的2个,未知功能蛋白1个。F172中参与自由基清除的1个,参与光合作用的2个,参与细胞生长和分裂的2个,参与基础代谢的4个,参与信号转导的2个,参与蛋白加工的1个,未知功能蛋白2个,其中22 k D干旱诱导蛋白的丰度明显提高,而在YL6中则检测不到此蛋白。这说明在干旱胁迫下抗旱性不同的甘蔗品种在蛋白质组成上有很大差异,推测这是不同甘蔗品种间抗旱性差异的重要分子基础。 Barrel method was used to analyze the difference protein between two drought-resistant F172 and drought-resistant YL6 sugarcane varieties under severe drought stress at the seedling stage, and 28 and 20 differences were found respectively by using two-dimensional electrophoresis Protein spots, some of which showed up-regulated expression, some showed down-regulated expression, and some newly added protein spots showed different performance due to different resistances. The differential proteins in F172 mainly showed up-regulated expression, whereas most of the expression in YL6 To down-regulate expression. Under severe drought stress, the variation of protein abundance of sugarcane varieties with different drought resistance were significantly different. The differential proteins were identified by MALDI-TOF-TOF / MS. The amino acid sequences of 18,14 proteins were identified respectively from YL6 and F172. The identified proteins were classified into 8 groups according to their function. YL6 participated in 2 free radicals scavenging, 6 involved in photosynthesis, 1 involved in cell growth and division, 6 involved in basal metabolism, 2 involved in defense response, and 1 unknown functional protein. F172 participate in the free radical scavenging 1, 2 involved in photosynthesis, involved in cell growth and division of 2, involved in the basic metabolism of 4, involved in signal transduction 2, involved in protein processing 1, unknown function There were 2 proteins, of which 22 kD drought-induced protein abundance was significantly increased, whereas in YL6 this protein was undetectable. This indicates that there are great differences in the protein composition of different drought-resistant sugarcane varieties under drought stress, suggesting that it is an important molecular basis for the differences in drought resistance among different sugarcane varieties.