从人胼胝、鸡胗、猪眼晶体、牛大脑及牛脊髓中提取角蛋白、结蛋白、波形蛋白、胶质纤维酸性蛋白和神经微丝蛋白，经分子量测定、电镜负染色观察确定为中间丝后，免疫ＢＡＬＢ／ｃ小鼠，用Ｂ淋巴细胞杂交瘤技术制备相应的单克隆抗体。经染色体分析、Ｉｇ亚类鉴定、免疫印迹及３８６例次人体肿瘤的免疫组化检测，肯定了它们是一组可用于临床病理诊断的中间丝系列单克隆抗体。 Keratin, desmin, vimentin, glial fibrillary acidic protein and neurofilament protein were extracted from callus, chicken gizzard, pig’s eye crystal, bovine brain and bovine spinal cord. The molecular weight and electron microscope negative staining were used to identify the intermediate silk BALB / c mice were immunized and the corresponding monoclonal antibodies were prepared using B lymphocyte hybridoma technique. Chromosome analysis, Ig subclass identification, immunoblotting and immunohistochemistry in 386 human tumors confirmed that they are a group of intermediate silk monoclonal antibodies that can be used in clinical and pathological diagnosis.