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目的：研究 Ｈ Ｉ Ｖ１ｐ２４ ｇａｇ 与 ｈ Ｉ Ｌ２基因在重组痘苗病毒中的共表达。方法：以痘苗病毒复制非必需区血凝素（ Ｈ Ａ）基因为侧翼，将编码人白细胞介素２（ｈ Ｉ Ｌ２）的基因片段克隆到真核表达质粒ｐ１６ Ｑ Ｆ２４ Ｉ Ｌ２，经同源重组和血凝素阴性空斑筛选，获得了重组痘苗病毒ｖ１６ Ｑ Ｆ２４ Ｉ Ｌ２。结果：经间接免疫荧光试验、 Ｄｏｔ Ｅ Ｌ Ｉ Ｓ Ａ 和 Ｗ ｅｓｔｅｒｎ ｂｏｌｔ 等检测证明，重组病毒能同时表达ｐ２４ ｇａｇ 蛋白和 Ｉ Ｌ２蛋白，表达产物分子量分别为２４ ０００、１６ ０００。结论：这种重组痘苗病毒人表达外源蛋白的成功，为获得更强的免疫效果，研制 Ｈ Ｉ Ｖ 重组病毒活疫苗提供一种安全的新途径。
Objective: To study H I V 1p24 gag and h I L 2 gene co-expression in recombinant vaccinia virus. Methods: The non-essential hemagglutinin (H A) gene was duplicated with vaccinia virus, and the gene fragment encoding hIL-2 was cloned into the eukaryotic expression plasmid p16 Q F24-I L2. After homologous recombination and hemagglutinin negative plaque screening, recombinant vaccinia virus v16 Q F24 I L2 was obtained. Results: Indirect immunofluorescence test, Dot-ELISA and Western bolt test showed that the recombinant virus can express p24 gag protein and I L 2 protein, the expression product molecular weight were 24 000,16 000. Conclusion: The successful recombinant vaccinia virus expressing human exogenous protein has provided a new safe way to develop a recombinant live vaccine of HIV.