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应用流式细胞仪观察两种固定剂对细胞荧光强度的影响。共分析了食管癌和肺癌各28份样品,结果表明:进行细胞DNA定量分析时,最好不用甲醛固定,而以乙醇固定为好,但不应超过3天。
The effects of two fixatives on the fluorescence intensity of cells were observed by flow cytometry. A total of 28 samples of esophageal cancer and lung cancer were analyzed. The results showed that it is better not to use formaldehyde to fix DNA but to fix it with ethanol, but it should not exceed 3 days.