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目的:探讨siRNA MK转染对乳腺癌MCF-7细胞增殖和转移能力的影响。方法:构建特异性抑制Midkine基因siRNA片段,转染至乳腺癌MCF-7细胞中,CCK-8法比较细胞增殖能力的改变,体外迁移实验比较细胞运动能力的改变,基质胶粘附实验比较细胞粘附率的变化。结果:MK表达下调后,MCF-7细胞的增殖能力明显降低(P<0.05),体外迁移能力显著降低(P<0.05),siRNA细胞对基质胶的粘附率明显降低(P<0.05)。结论:MK基因表达下调能降低乳腺癌细胞迁移运动和降低细胞对基质胶的粘附率,并降低肿瘤细胞的增殖,提示MK在乳腺癌恶性进展中起重要作用。
Objective: To investigate the effect of siRNA MK transfection on the proliferation and metastasis of breast cancer MCF-7 cells. Methods: The Midkine gene specific siRNA fragment was constructed and transfected into MCF-7 breast cancer cells. CCK-8 method was used to compare the changes of cell proliferation. The in vitro migration assay was used to compare the changes of cell motility. Change in adhesion rate. Results: The proliferation of MCF-7 cells was significantly decreased (P <0.05) and the ability of migration in vitro was significantly decreased (P <0.05). The adhesion rate of MCs to Matrigel was significantly decreased (P <0.05). Conclusion: The down-regulation of MK gene expression can reduce the migration of breast cancer cells and reduce the cell adhesion rate to Matrigel, and reduce the proliferation of tumor cells, suggesting that MK plays an important role in the malignant progression of breast cancer.