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为了克隆凤冈野生天麻的抗真菌肽基因(gaf),以凤冈野生天麻的RNA为模板,采用RT-PCR技术扩增抗真菌肽基因(gaf),经克隆和测序结果显示:RT-PCR产物电泳扩增的目标条带长度为545 bp;经测序和序列分析,该基因与Genebank中公布的序列编码天麻抗真菌蛋白的基因序列(AY032588)同源性达97%。此研究成功地克隆了凤冈野生天麻抗真菌肽编码基因。
In order to clone the antifungal peptide gene (gaf) of wild Gastrodia elata Blume, the antifungal peptide gene (gaf) was amplified by RT-PCR using the RNA of Phyllostachys pubescens as a template. The results of cloning and sequencing showed that RT-PCR The length of the target amplified by electrophoresis was 545 bp. The sequence of this gene was 97% homologous to the gene sequence (AY032588) of Gene Fungus encoded by Genebank. This study successfully cloned Fenggang wild gastrodia antifungal peptide encoding genes.