论文部分内容阅读
细胞因子在原核系统中高效稳定地表达,除了需强启动子外,mRNA的结构以可在转录后水平影响其翻译效率,国外学者研究表明:mRNA起始密码下游序列并不是随机分布的,将序列沉默突变后,不同序列的翻译效率并不一样,可能mRNA分子与核糖体16s rRNA结合时,除了SD区和起始密码外,SD上游和起始码下游序列也参与了16s rRNA间的结合.通过对T7噬菌体基因的研究证实:若起始密码下游存在16s rRNA 3’端+1469～+1482区的匹配序列,该基因即可高效表达.将我室曾克隆表达过的细胞因子的编码序列用
In addition to the strong promoters, the structure of mRNA can affect the translation efficiency at the post-transcriptional level. Foreign scholars have shown that the downstream sequences of mRNA initiation codons are not randomly distributed. After sequence silent mutation, the translation efficiency of different sequences is not the same. It is possible that in combination with the ribosomal 16s rRNA, the mRNAs of SD and start codon, besides the SD region and the start codon, are also involved in the 16s rRNA binding Through the study of T7 phage gene, it was confirmed that the gene could be efficiently expressed if there was a matching sequence of 3 ’end of 16s rRNA + 1469 ~ + 1482 region downstream of the start codon.According to the coding of cytokines expressed in our laboratory Sequence used