利用基于扫描相机的荧光寿命成像显微系统,以细胞周期为模型,研究转染绿色荧光蛋白的HeLa细胞的荧光寿命。结果表明,处于周期内不同进程的细胞的荧光寿命为2.50~3.00 ns。处于分裂期的细胞的荧光寿命在1 h内从2.86 ns下降到2.82 ns;在DNA合成前期的8 h内,荧光寿命从2.82 ns下降到2.78 ns。荧光寿命的差异反映了细胞周期中核浆内大分子浓度的变化,对了解细胞周期的分子机制有一定的意义。 Fluorescence lifetime of HeLa cells transfected with green fluorescent protein (GFP) was studied by using the cell-cycle-based fluorescence lifetime imaging microscopy system. The results showed that the fluorescence lifetime of cells with different processes in the cycle was 2.50 ~ 3.00 ns. Fluorescence lifetime of cells in mitosis decreased from 2.86 ns to 2.82 ns in 1 h; fluorescence lifetime decreased from 2.82 ns to 2.78 ns within 8 h of DNA synthesis. The difference in fluorescence lifetime reflects the change of macromolecule concentration in the nucleus pulposus during the cell cycle, which is of certain significance for understanding the molecular mechanism of the cell cycle.