痰的结核菌检验在结核病防治工作中具有重要的意义。涂片法查痰,是检验痰内结核菌既简便又经济的方法。过去我们检验室用的是萋尼(Ziehl—Neelsen)氏法,因为此法所取痰液标本有限,而且粘稠度较大的痰又不易在玻片上划匀,当每毫升痰内结核杆菌数少于5～10万时,则不易检出,所以阳性检出率不 Mycobacterium tuberculosis test is of great significance in the prevention and treatment of tuberculosis. Smear sputum smear, sputum is to check the tuberculosis is both simple and economical method. In the past, we used the Ziehl-Neelsen method in our laboratory. Because sputum samples were limited and the viscous sputum was not easily smoothed on the slide. When each ml of Mycobacterium tuberculosis Number of less than 5 to 10 million, it is not easy to detect, so the positive detection rate is not