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目的:探讨三七总皂甙(PNS)的脑保护作用机制.方法:体外培养鸡胚脑神经细胞,用氰化钠造成缺氧,作为试验PNS作用的模型,细胞内三磷酸腺苷(ATP)和培养液中肌酸激酶浓度作为观察指标,分别用HPLC-UV和全自动生化分析仪定量.结果:缺氧前30min将PNS 50和100 mg L~(-1)加入到培养液中能明显延缓缺氧2h细胞内ATP的耗竭(分别为11.3±1.5和12.8±2.2μmol/g protein),促进再给氧30min时细胞内ATP的恢复(分别为21.0±2.0和22.7±2.6 μmol/g protein).PNS于缺氧开始或再给氧时给予,仍能促进再给氧期细胞内ATP的恢复,减少神经细胞内肌酸激酶的释放. 结论:PNS对培养神经细胞缺氧性损伤具有保护作用,其机制可能与改善能量代谢,保护细胞结构完整性有关.
OBJECTIVE: To investigate the mechanism of brain protection of panax notoginseng saponins (PNS). Methods: In vitro culture of embryonated neurons in chicken embryos, hypoxia induced by sodium cyanide, as a model to test the effects of PNS, intracellular adenosine triphosphate (ATP) and broth The concentration of creatine kinase was used as an observation index, and was quantified by HPLC-UV and automatic biochemical analyzer. Results: The addition of PNS 50 and 100 mg L~(-1) to the culture medium for 30 min before hypoxia could significantly delay hypoxia. The intracellular ATP depletion at 2h (11.3±1.5 and 12.8±2.2μmol/g protein, respectively) promoted intracellular ATP recovery at 30 min after reoxygenation (21.0±2.0 and 22.7±2.6 μmol/g protein, respectively). PNS The administration of hypoxia or reoxygenation can still promote the recovery of intracellular ATP during reoxygenation and reduce the release of creatine kinase in neurons. Conclusion: PNS has a protective effect on cultured neurons against hypoxia. The mechanism may be related to improving energy metabolism and protecting cell structural integrity.