A rapid and practical method for direct detection of lisinopril in anion exchange chromatography(AEC) has been developed with integrated pulsed amperometric detection(IPAD).Dionex AS 18(250 mm×2 mm) and AG 18(50 mm×2 mm) columns and 40 mmol/L NaOH solution were used for separation.Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio(S/N).Utilizing the optimized waveform,the repeatability(intra-day) precision and intermediate(inter-day) precision were obtained with relative standard deviation(RSD) of 0.74,0.93,respectively.The limit of quantification(LOQ) and limit of detection(LOD) were found to be 0.37,0.12ng/mL,respectively,with the correlation coefficient of 0.9998 over concentration range 0.01-1μg/mL.The present method was successfully applied to the determination of lisinopril in human plasma.The recoveries of plasma sample spiked by 0.2μg/mL,0.8μg/mL lisinopril were 98.31-103.23%with RSD of 1.41%, 0.61%,respectively. A rapid and practical method for direct detection of lisinopril in anion exchange chromatography (AEC) has been developed with integrated pulsed amperometric detection (IPAD). Dionex AS 18 (250 mm × 2 mm) and AG 18 (50 mm × 2 mm) columns and 40 mmol / L NaOH solution were used for separation. Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio (S / N). Utilizing the optimized waveform, the repeatability (intra-day) precision and intermediate respectively. The limit of quantification (LOQ) and limit of detection (LOD) were found to be 0.37, 0.12 ng / mL, respectively, with the correlation coefficient of 0.9998 over concentration range 0.01-1 μg / mL. The present method was successfully applied to the determination of lisinopril in human plasma. The recoveries of plasma sample spiked by 0.2 μg / mL, 0.8 μg / mL lisinopril were 98.31-103.23 % with RSD of 1.41%, 0.61%, respectively.