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作者报道了间日疟原虫红内期体外培养43天的结果。从曾旅居于巴西6个月的27岁年轻人采得间日疟原虫虫株,原虫密度为2.5%(大滋养体及裂殖体),用Tragcr烛缸平皿法,培养液由RPMI 1640,葡萄糖及Rh阳性的O型血清组成,不同点主要是在培养液的pH方面。在培养的头6天pH较酸,每天更换3次培养液,每2天添加红细胞1次。间日疟原虫连续培养27天,然后将其冻存于液氮中;为测定原虫活力,再将冻存的原虫进行复苏培养。到43天时中止培养再冻存于液氮。在培养过程中可见到大滋养体,未成熟裂殖体,在39天时还见到幼小滋养体及环状体。在中止培养时,原虫密度为0.25‰。
The authors reported the results of in vitro culture of Plasmodium vivax for 43 days in vitro. Plasmodium vivax strains were collected from a 27-year-old young man who had lived in Brazil for six months and had a protozoan density of 2.5% (trophozoites and schizonts) using a Tragcr candle jar method. The culture consisted of RPMI 1640, Glucose and Rh-positive O-type sera, the difference is mainly in the pH of the culture medium. During the first 6 days of culture, the pH was more acidic, and the medium was changed 3 times a day. Erythrocytes were added every 2 days. Plasmodium vivax was continuously cultured for 27 days and then frozen in liquid nitrogen; in order to determine the viability of the protozoa, the frozen protozoa were resuscitated and cultured. By 43 days, culture was resumed and stored in liquid nitrogen. In the training process can be seen in large trophozoites, immature schizonts, 39 days also see the young trophozoites and the ring. Protel parasite density was 0.25 ‰ at the time of suspension.