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T0135是2009年从中籼3037第11染色体短臂端三体(2n+11S·)自交后代中发现的形态变异株.选用第11号染色体着丝粒区域的特异分子细胞学标记5S rDNA进行染色体荧光原位杂交(fluorescent in situ hybridization,FISH)分析,发现T0135是第11染色体短臂双着丝粒染色体.为了研究该双着丝粒染色体有性繁殖过程中的遗传稳定性,对T0135有性繁殖后代进行FISH分析,观察后代中染色体类型并进行统计,发现后代染色体类型与亲本类型相同的占94%,即双着丝粒染色体能进行正常的减数分裂,通过着丝粒特异组蛋白(centromere specific histone H3,CENH3)免疫检测确定在双着丝粒染色体中只有一个有CENH3信号,说明该双着丝粒材料中只有一个行使正常功能的着丝粒,另外一个着丝粒处于失活状态.
T0135 is a morphological variant found in the selfing offspring of short arm end trisomic (2n + 11S ·) on chromosome 11 of Zhongnkan 3037 in 2009. Chromosomes were labeled with specific molecular cytology of chromosome 11 on chromosome 11 Fluorescence in situ hybridization (FISH) analysis showed that T0135 was a short-arm dicentric chromosome on chromosome 11. In order to study the genetic stability during sexual reproduction of this dicentric chromosome, FISH analysis of breeding offspring to observe the type of chromosomes in the offspring and statistical and found that the offspring of the same type of chromosome type and parental 94%, that the dicentric chromosome normal meiosis, through centromere specific histone ( centromere specific histone H3, CENH3) Immunoassay confirmed that there was only one CENH3 signal in the dicentric chromosome, indicating that only one of the dicentric materials functioned normally, while the other centromere was inactivated .