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目的 了解非血缘关系骨髓移植后慢性移植物抗宿主病 (cGVHD)患者的T细胞受体(TCR)VβT细胞的分布和克隆性。方法 应用逆转录 聚合酶链反应 (RT PCR)扩增 1例非血缘关系骨髓移植后cGVHD患者的外周血单个核细胞的TCRVβ 2 4个亚家族的CDR3,了解患者TCRVβT细胞的分布情况 ,PCR产物进一步经基因扫描分析确定T细胞克隆性。结果 患者存在 8个TCRVβ亚家族T细胞 ;其中Vβ2、Vβ3、Vβ8和Vβ13亚家族为克隆性增殖T细胞。 结论 患者外周血出现不均匀性 (即倾斜性 )分布和克隆性生长TCRVβ家族T细胞 ,它可能与cGVHD的发生有关。
Objective To investigate the distribution and clonality of T cell receptor (TCR) Vβ T cells in patients with chronic graft-versus-host disease (cGVHD) after non-blood relationship bone marrow transplantation. Methods RT-PCR was used to amplify the CDR3 of TCRVβ2 4 subfamilies of peripheral blood mononuclear cells in 1 unrelated cGVHD patients after bone marrow transplantation to understand the distribution of TCRVβT cells. PCR products T cell clonality was further confirmed by gene scanning analysis. Results There were 8 TCRVβ subfamily T cells in patients. Vβ2, Vβ3, Vβ8 and Vβ13 subfamilies were clonal proliferating T cells. Conclusion The distribution of TCRVβ family T cells in peripheral blood of patients with non-uniform distribution (ie, tilt) and clonal growth may be related to the occurrence of cGVHD.