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目的确认长春地区是否莱姆病疫源地。方法用酶联免疫吸附试验等方法检测莱姆病人群特异IgG、IgM抗体,全沟硬蜱中肠伯氏疏螺旋体培养。长春地区全沟硬蜱733只,用BSK33℃培养,获得伯氏疏螺旋体培养物278株,用兔抗美国B31特异性单克隆抗体和针对莱姆病螺旋体种属特异M7,间接免疫荧光抗体进行试验。其结果与美国菌株稍有差异,同国内分离株M7相同。血清学调查298人份,15人阳性,平均感染率为5.03%。结果长春地区莱姆病疫源人群、家畜、动物感染莱姆病。结论首次确认长春地区存在莱姆病自然疫源地。
Objective To confirm whether Lyme disease origin in Changchun area. Methods Enzyme-linked immunosorbent assay was used to detect Borrelia burgdorferi in Lyme disease population-specific IgG and IgM antibodies. 733 ticks of Ictalurus punctatus in Changchun area were cultured with BSK at 33 ℃, and 278 strains of Borrelia burgdorferi were obtained. Rabbit anti-B31 specific monoclonal antibody and specific M7 against Lyme disease spirochetes were used for indirect immunofluorescent antibody test. The result is slightly different from the U.S. strain, which is the same as the domestic isolate M7. Serological survey of 298 people, 15 were positive, the average infection rate was 5.03%. Results Lyme disease population, livestock and animals in Changchun were infected with Lyme disease. Conclusion It is the first time to confirm the presence of natural Lyme disease in Changchun.